Silver staining technique and kit

Details Silver staining technique and kit Silver staining technique and kit

1989-09-12

1991-11-12

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Optical result

422 61, 436 86, 436 94, 436169, 436905, G01N 2177, G01N 3368

Patent

active

050647687

ABSTRACT:
An improved silver staining technique is disclosd for the detection of a biocomponent contained in a support medium. The technique includes fixing the support medium with a fixing agent, pretreating the thus-fixed support medium with a sensitizer, treating the resultant support medium with a silver staining solution and then developing the thus-treated support medium with a reducing agent. In one aspect of the invention, thiourea is incorporated in either one or both of the fixing agent and sensitizer. In another aspect of the invention, thiosulfate is contained in the reducing agent. According to the invention, improved stainability and contrast are obtained. Also disclosed is a silver staining kit.

REFERENCES:
patent: 4468466 (1984-08-01), Morrissey
patent: 4554254 (1985-11-01), Krystal
patent: 4575452 (1986-03-01), Lee et al.
patent: 4582808 (1986-04-01), Oosawa et al.
patent: 4690901 (1987-09-01), Giammara et al.
patent: 4703016 (1987-10-01), Merril
Giulian et al, Anal. Biochem., vol. 129, No. 2, pp. 277-287, 1983.

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