Silver staining method

Details Silver staining method Silver staining method

1984-05-18

1986-04-15

Turk, Arnold

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436169, 436177, G01N 2178, G01N 3368

Patent

active

045828083

ABSTRACT:
A silver staining method comprising the steps of (1) pretreating a carrier bearing thereon a substance to be detected with an alcoholic solution containing polyethylene glycol or polyoxyethylene alkylphenol, and (2) treating the pretreated carrier with a solution comprising silver nitrate, a compound of R--NH.sub.2, and a caustic alkali in ratios by molecular weight of 1:4.5-9.5:1.0-26.0.
The method according to the invention involves advantages of high sensitivity and good reproducibility of detection, simple operation, safe handling etc.

REFERENCES:
patent: 3997656 (1976-12-01), Wertlake et al.
patent: 4416998 (1983-11-01), Adams et al.
patent: 4468466 (1984-08-01), Morrissey
Ohsawa et al., Analytical Biochemistry, 135, 409-415 (1983).
Palumbo et al., Analytical Biochemistry, 134, 254-258 (1983).
Wayne Wray et al, "Silver Staining of Proteins in Polyacrylamide Gels", (Nov. 15, 1981), pp. 197-203 (Analytical Biochemistry).
Sammons et al, "Ultrasensitive Silver Based Color Staining of Polypeptides in Polyacrylamide Gels", (Feb. 1981), pp. 135-141, (Electrophoresis, vol. 2, no. 1).

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