Silver stain for rapid, quantitative detection of polypeptides a

Details Silver stain for rapid, quantitative detection of polypeptides a Silver stain for rapid, quantitative detection of polypeptides a

1986-05-05

1987-10-27

Richman, Barry S.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

422 61, 436 94, 436169, 436174, 436905, G01N 2177, G01N 3352, G01N 3358

Patent

active

047030160

ABSTRACT:
A simple, positive image forming silver stain which takes less than 15 minutes to perform is disclosed for the detection of nanogram quantities of proteins and DNA on membranes and thin layer plates. This stain demonstrates a reproducible curvilinear relationship between silver density and the amount of protein or DNA, over an averaged concentration range from 1 nanogram to 300 nanograms for proteins and 10 nanograms to 700 nanograms for DNA. The ease of staining proteins and DNA on membranes, combined with the stain's sensitivity and reproducibility, permits quantitative determination and assay of proteins and DNA.

REFERENCES:
patent: 4405720 (1983-09-01), Merril
patent: 4416998 (1983-11-01), Adams et al.
patent: 4434234 (1984-02-01), Adams et al.
patent: 4554254 (1985-11-01), Krystal
patent: 4555490 (1985-11-01), Merril
patent: 4575452 (1986-03-01), Lee et al.
Merril et al., Anal. Biochem., vol. 110, No. 1, pp. 201-207, 1981.
Yuen, et al., (1982), Anal. Biochem., 126:398-402, "A Silver-Staining Technique for Detecting Minute Quantities of Proteins on Nitrocellulose Paper: Retention of Antigenicity of Stained Proteins".
Moeremans, et al., (1985), Anal. Biochem, 145:315-321, "Sensitive Colloidal Metal (Gold or Silver) Staining of Protein Blots on Nitrocellulose Membranes".
Merril et al., 1982, Electrophoresis, 3:17-23.
Merril et al., 1984, Electrophoresis, 5:289-297.

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