Silver stain for collagen matrix from paraffin embedded tissue

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving fixed or stabilized – nonliving microorganism,...

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C12Q 108

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active

057006563

ABSTRACT:
A technique for staining and preparing mammalian tissue for examination and study is disclosed. The technique features a fixed tissue that is embedded with paraffin to facilitate the cutting of uniformly thin sections and the penetration of a silver stain into the interstices of the collagen matrix. In particular, a 25-30 micron thick slice of cardiac tissue is silver stained and examined for cardiomyopathies.

REFERENCES:
Novotny E. et al., "A Modification of the Glees Silver Impregnation for Normal and Degenerating Nervous Tissue", Stain Tech. 49(5):273-80 (1974).
Moos T et al., Histochemistry 99(6):471-5 (1993).
Brown GG. et al., Stain Technology 44(5):247-9 (1969).
Abstract 151 Interstitial Connective Tissue Abnormalities in Feline Cardiomyopathies, Si-Kwang Liu et al. United States & Canadian Academy of Pathology Annual Meeting, San Francisco Mar. 12-18, 1994.

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