Silver methenamine staining method

Chemistry: analytical and immunological testing – Peptide – protein or amino acid

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424 3, 436 63, 436 87, 436 94, 436164, G01N 2175, G01N 3352

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active

046909010

ABSTRACT:
A staining technique for specimens which involves the sequential treatment of specimens with periodic or hydrochloric acid, thiocarbohydrazide or thiosemicarbazide, and silver methenamine. The technique, when using periodic acid, provides an excellent stain to evaluate glycomacromolecules and fibrovascular tissue and to conduct a broad spectrum of staining procedures for all modes of microscopy. Use of hydrochloric acid facilitates evaluation of cell nuclear DNA and chromatin.

REFERENCES:
patent: 4296201 (1981-10-01), Ax et al.
Merril et al., Anal. Biochem., vol. 110, No. 1, pp. 201-207, 1981.
Grocott, Am. J. Clin. Path., vol. 25, pp. 975-979, 1955.
Thiery, Chemical Abstract 68: 112091R, 1968.
Kohler, Chem. Abs., 94: 135465s, 1981.
Burr, Chem. Abs., 77: 45161;, 1972.
Harada, Chem. Abs., 93: 110148g, 1980.
Burr, Chem. Abs., 79: 15460e, 1973.
Berger et al, Chem. Abs. 79: 89920b, 1973.
Sedar et al, Chem. Abs., 80: 142697h, 1974.
Dutt, Chem. Abs., 91: 206787z, 1979.
Dutt, Chemical Abstract, 90: 117461a, 1978.
Courtoy et al, Chem. Abs., 88: 148459u, 1978.
Arnold M. Seligman, Hannah L. Wasserkrug and Jacob S. Hanker, "A New Staining Method (OTO) for Enhancing Contrast of Lipid-Containing Membranes and Droplets in Osmium Tetroxide-Fixed Tissue with Osmiophilic Thiocarbohydrazide (TCH)", reprinted from The Journal of Cell Biology, 1966, vol. 30, No. 2. pp. 424-432.
Jacob S. Hanker, Chandicharan Deb, Hannah L. Wasserkrug and Arnold M. Seligman, "Staining Tissue for Light and Electron Microscopy by Bridging Metals with Multidentate Ligands", reprinted from Science, Jun. 17, 1966, vol. 152, No.l 3729, pp. 1631-1634.
Hanker et al, "Osmiophilic Reagents: New Cytochemical Principle for Light and Electron Microscopy"-Science-Nov. 20, 1964 vol. 146, No. 3647, pp. 1039-1043.
"Histochemical Demonstration of Some Oxidized Macromolecules with Thiocarbohydrazide (TCH) or Thiosemicarbazide (TSC) and Osmium Tetroxide," The Journal of Histochemical and Cytochemistry, 1965, Seligman and Hanker.
"Importance of Controls for the Demonstration of Carbohydrates in Electron Microscopy with the Silver Methenamine or the Thiocarbohydrazide-Silver Proteinate Methods",-Aug. 1, 1973, R. Courtoy.
"Mise en Evidence des Polysaccharides sur Coupes Fines en Microscopie Electronique," (1967) Thiery-J. Microscopie (1967), 6, 987-1018.
"New Electron Opaque and Conductive Stains for Basement Membranes", AADR Abstracts-1983-B. Giammara, T. Romaine, and J. Hanker.

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