Chemistry of inorganic compounds – Phosphorus or compound thereof – Oxygen containing
Patent
1993-09-27
1996-04-02
Robinson, Douglas W.
Chemistry of inorganic compounds
Phosphorus or compound thereof
Oxygen containing
423325, 4233281, 423344, 536 254, C01B 3302, C07H 106
Patent
active
055038166
ABSTRACT:
The present invention relates to a silicon-containing material which exhibits sufficient hydrophilicity and sufficient electropositivity to bind DNA from a suspension containing DNA and permit elution of the DNA from the material. Generally, the hydrophilic and electropositive characteristics are expressed at the surface of the silicon-containing material. Preferred silicon-containing materials of the present invention include boron silicate, aluminum silicate, phosphosilicate, silica carbonyl, silica sulfonyl and silica phosphonyl. The silicon-containing materials of the present invention are particularly useful in processes for purification of DNA from other cellular components. In these processes, a suspension of cellular components is placed in contact with the silicon-containing material, the silicon-containing material is washed to remove all cellular components other than DNA which are bound to the material, and the bound DNA is eluted from the material. Several of the silicon-containing materials are capable of binding and eluting DNA using only water.
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Down James A.
Howard Adriann J.
Woodard Daniel L.
Becton Dickinson and Company
Highet David W.
Kunz Gary L.
Robinson Douglas W.
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