Signalling compounds and methods for detecting hydrogen...

Chemistry: analytical and immunological testing – Optical result – With fluorescence or luminescence

Reexamination Certificate

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C436S546000, C436S904000, C435S028000, C568S001000, C549S213000

Reexamination Certificate

active

07390670

ABSTRACT:
Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula:is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig—OH or Sig—O−is produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.

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