Radiant energy – Luminophor irradiation
Patent
1997-10-27
1999-11-09
Westin, Edward P.
Radiant energy
Luminophor irradiation
2504591, 356317, G01N 2164
Patent
active
059819570
ABSTRACT:
The present invention additionally comprises a method and apparatus for generating and mixing signals for frequency-domain lifetime and spectral fluorometry. The present invention comprises a plurality of signal generators that generate a plurality of signals where the signal generators modulate the amplitude and/or the frequency of the signals. The present invention uses one of these signals to drive an excitation signal that the present invention then directs and transmits at a target mixture, which absorbs the energy from the excitation signal. The property of fluorescence causes the target mixture to emit an emitted signal that the present invention detects with a signal detector. The present invention uses a plurality of mixers to produce a processor reference signal and a data signal. The present invention then uses a processor to compare the processor reference signal with the data signal by analyzing the differences in the phase and the differences in the amplitude between the two signals. The processor then extracts the fluorescence lifetime and fluorescence spectrum of the emitted signal from the phase and amplitude information using a chemometric analysis.
REFERENCES:
patent: 5196709 (1993-03-01), Berndt et al.
Weber, G., Resolution of the Fluorescence Lifetimes in a Heterogeneous System by Phase and Modulation Measurements, J.Phys. Chem., 85, (1981) pp. 949-953.
Lackowicz, J.R., Analysis of Fluorescence Decay Kinetics from Variable-Frequency Phase Shift and Modulation Data, Biophys. J., 46 (1984) pp. 463-477.
Burdick, D.S. et al., Resolution of Multicomponent Fluorescent Mixtures by Analysis of the Excitation-Emission-Frequency Array, J. Chemometrics, 4 (1990) pp. 15-28.
Chin Robert C.
Cruce Tommy C.
Hallidy William H.
Booth Matthew J.
Hanig Richard
Systems&Processes Engineering Corporation
Westin Edward P.
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