Signal enhancement system with multiple labeled-moieties

Details Signal enhancement system with multiple labeled-moieties Signal enhancement system with multiple labeled-moieties

2011-07-05

2011-07-05

Nguyen, Bao-Thuy L (Department: 1641)

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

C435S287100, C435S810000, C422S420000, C422S425000, C422S430000, C977S700000, C977S773000, C977S788000, C977S918000

Reexamination Certificate

active

07972837

ABSTRACT:
Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.

REFERENCES:
patent: 4434150 (1984-02-01), Azad et al.
patent: 4943522 (1990-07-01), Eisinger et al.
patent: 5073484 (1991-12-01), Swanson et al.
patent: 5089423 (1992-02-01), Diamandis et al.
patent: 5188937 (1993-02-01), Schulte et al.
patent: 5252459 (1993-10-01), Tarcha et al.
patent: 5770460 (1998-06-01), Pawlak et al.
patent: 5773234 (1998-06-01), Pronovost et al.
patent: 5895750 (1999-04-01), Mushahwar et al.
patent: 6096563 (2000-08-01), Hajizadeh et al.
patent: 6146589 (2000-11-01), Chandler
patent: 6803196 (2004-10-01), Lyon et al.
patent: 7270995 (2007-09-01), Matsushita et al.
patent: 7776617 (2010-08-01), Lee et al.
patent: 2003/0143639 (2003-07-01), Matsushita et al.
patent: 2004/0137493 (2004-07-01), Goldberg et al.
patent: 0258963 (1988-03-01), None
patent: 0354847 (1990-02-01), None
patent: 0 387 027 (1990-09-01), None
patent: 0813064 (1997-12-01), None
patent: WO 94/11734 (1994-05-01), None
patent: WO96/19731 (1996-06-01), None
patent: WO96/24060 (1996-08-01), None
patent: WO98/18488 (1998-05-01), None
patent: WO98/36278 (1998-08-01), None
patent: WO 99/58948 (1999-11-01), None
patent: WO00/25135 (2000-05-01), None

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