Signal enhancement method and kit

Details Signal enhancement method and kit Signal enhancement method and kit

1996-10-21

1998-12-29

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 243, 935 76, 935 77, 935 78, C12Q 168, C12N 1500, C07H 2102, C07H 2104

Patent

active

058539930

ABSTRACT:
The invention discloses and claims a signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence. The method comprises (a) contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each probe including a signal region and an oligonucleotide sequence which is complementary to, and capable of forming a stable hybrid with the analyte homopolymeric region, whereby the hybridization of multiple reporter probes to the homopolymeric region provides for signal amplification; and (b) forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions.

REFERENCES:
patent: 4563419 (1986-01-01), Ranki et al.
patent: 4751177 (1988-06-01), Stabinsky
patent: 4882269 (1989-11-01), Schneider et al.
patent: 4894325 (1990-01-01), Englehardt et al.
patent: 5124246 (1992-06-01), Urdea et al.
patent: 5437977 (1995-08-01), Segev
patent: 5487973 (1996-01-01), Nilsen et al.
patent: 5561043 (1996-10-01), Cantor et al.
patent: 5627030 (1997-05-01), Pandian et al.
patent: 5629156 (1997-05-01), Shah et al.
patent: 5695936 (1997-12-01), Mandrand et al.
Vo, Thuong et al., "Targeting Pyrimidine Single Strands by Triplex Formation: Structural Optimization of Binding", Nucleic Acids Research, vol. 23, No. 15, 1995, pp. 2937-2944.
Matthews et al., Analytical Biochemistry 169: pp. 1-25 (1988).
Frazen et al., Lancet 10 Mar. 1984: pp. 525-527 (1984).
Chen et al., Molecular and Biochemical Parasitology 44: pp. 165-174 (1991).
Palva et al., Gene 15 :43-51 (1981).

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