Signal enhancement in immunoassay by modulation of chemical cata

Details Signal enhancement in immunoassay by modulation of chemical cata Signal enhancement in immunoassay by modulation of chemical cata

1986-05-12

1989-03-07

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 18, 435 19, 435 21, 435810, 436518, 436537, 436808, 436821, G01N 3353, G01N 33543, G01N 33542, C12Q 144

Patent

active

048106313

ABSTRACT:
A method for immunoassay for a ligand suspected to be present in a fluid includes use of an enzyme, a metal ion catalyst for an indicator reaction and a blocked modulator for the catalyst. Ligand present in the fluid binds to an antiligand. The resulting bound fraction activates the enzyme to unblock the modulator. The free modulator activates or inhibits the catalyst thereby modulating the rate of an indicator reaction between a substrate and a redox reagent. The presence of absence of the ligand in the fluid is indicated by a signal, such as a color change or a rate of color change, consequent to the indicator reaction. The invention includes a kit of materials useful for performing the method of the invention.

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Dolmanova, I. et al., J. Anal. Chem. USSR, 32:638 (1977).
Antonov, V., et al., J. Anal. Chem. USSR, 31:168 (1976).

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