Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving glucose or galactose
Patent
1988-08-11
1990-10-09
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving glucose or galactose
435 4, 435 18, 435 19, 435 20, 435 21, 435 22, 435 23, 435 24, 435 25, C12Q 154, C12Q 148
Patent
active
049620240
ABSTRACT:
A method for assay for an unknown enzyme suspected to be present in a liquid includes signal amplification by use of a second enzyme and a blocked modulator for the second enzyme. Unknown enzyme in the liquid removes a blocking group from the blocked modulator. The resulting modulator activates or inhibits the second enzyme which catalyzes an indicator reaction in which a substrate is converted to a product. The presence or absence of the unknown enzyme in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the indicator reaction. The concentration of the enzyme in the sample may be determined by the measurement of the signal. The invention includes a kit of materials useful for performing the method of the invention. The method involves the hydrolyzing of a blocked fluoroketone inhibitor to facilitate the analytical method.
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Gelb et al., Fluoro Ketone Inhibitors of Hydrolytic Enzymes, Apr. 9, 1985, Biochemistry, vol. 24, No. 8, pp. 1813-1817.
Becton Dickinson and Company
Brown Richard E.
Rosen Sam
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