Signal amplifying cobalt (III) redox reagents and methods for th

Details Signal amplifying cobalt (III) redox reagents and methods for th Signal amplifying cobalt (III) redox reagents and methods for th

1986-07-28

1992-08-25

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4, 435 25, 435 26, 435 28, 435 29, 435 30, 436 84, 436169, 436170, 436904, 422 55, 422 56, 422 57, 430223, 430224, 430225, 556138, 556140, 556146, 556148, G01N 3122, G01N 3352, G01N 3350, C12Q 104

Patent

active

051418550

ABSTRACT:
Reagents and methods for the determination of analytes are disclosed, using water soluble cobalt(III) complexes and metallizable dyes to form cobalt(III) complexes of the metallizable dyes. Analytes which can be determined include enzymes, cells and biological reductants, such as NADH, NADPH and ascorbate. Amplification is provided since one equivalent of the analyte provides more than one equivalent of the detectable species.

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