Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving luciferase
Reexamination Certificate
2004-09-02
2011-10-18
Gitomer, Ralph (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving luciferase
C435S018000, C435S023000
Reexamination Certificate
active
08039228
ABSTRACT:
Described herein are zymogens, methods of use for zymogens, and devices that incorporate zymogens. The zymogens include a substrate and an enzyme. The substrate can inhibit the enzyme and is a target for a protein produced by a microorganism. When the substrate is modified by a protein produced by a microorganism, the enzyme is activated. The zymogens can be used to amplify detection assays.
REFERENCES:
patent: 5714389 (1998-02-01), Charlton et al.
patent: 5811252 (1998-09-01), Verheijen et al.
patent: 7566564 (2009-07-01), Colpas et al.
patent: 2003/0096315 (2003-05-01), Sanders
patent: 2006/0257955 (2006-11-01), Colpas et al.
patent: 2007/0122873 (2007-05-01), Colpas et al.
patent: 2002-159297 (2002-06-01), None
patent: WO 02/16634 (2002-02-01), None
patent: WO 02/082078 (2002-10-01), None
patent: WO 03/063693 (2003-08-01), None
patent: WO 03/065004 (2003-08-01), None
patent: WO 2004/047614 (2004-06-01), None
patent: WO 2004/087942 (2004-10-01), None
patent: WO 2005/042771 (2005-05-01), None
Enguita, F.J. et al., “Crystal Structure of a Bacterial Endospore Coat Component: A Laccase With Enhanced Thermostability Properties,”J. Biol. Chem., 278(21):19416-19425 (2003).
Plainkum, P., et al., “Creation of a Zymogen,”Nature Struct. Biol., 10(2):115-119 (2003).
Saghatelian, A., et al., “DNA Detection and Signal Amplification Via an Engineered Allosteric Enzyme,”J. Am. Chem. Soc., 125:344-345 (2003).
Verheijen, J.H., et al., “Modified Proenzymes as Artificial Substrates for Proteolytic Enzymes: Colorimetric Assay of Bacterial Collagenase and Matrix Metalloproteinase Activity Using Modified Pro-Urokinase,”Biochem. J., 323:603-609 (1997).
Jobin et al., “Identification and Characterization of Four Proteases Produced byStreptococcus suis,” FEMS Microbiol. Lett. 220:113-19 (2003).
Notice of Allowance in U.S. Appl. No. 11/595,275, Mailed Mar. 24, 2009.
Interview Summary and Examiner's Amendment in U.S. Appl. No. 11/595,275, Mailed Mar. 24, 2009.
Colpas Gerard J.
Sanders Mitchell C.
Sebastian Shite
Expressive Constructs, Inc.
Gitomer Ralph
Gough Tiffany
Hamilton Brook Smith & Reynolds P.C.
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