Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2008-07-29
2008-07-29
Chin, Christopher L (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C422S082110, C435S006120, C435S007920, C435S287200, C435S288700, C435S808000, C436S164000, C436S512000, C436S514000, C436S524000, C436S525000, C436S805000, C436S827000
Reexamination Certificate
active
07405054
ABSTRACT:
The present invention provides methods and compositions for amplifying the detection signal in surface plasmon resonance (SPR)-based flow systems. The signal amplification methods comprise the use of well established marker systems that provide a precipitate. The marker systems include, for example, enzyme and nucleation systems. Enzymes suitable for use as a marker system include peroxidases and phosphatases. The amplification system is useful in any SPR-based detection system including microfluidic systems, e.g., “lab on a chip” systems and the like. The methods can comprise any SPR-based assay format, including typical immunoassay formats. The immunoassay formats can include competitive and sandwich assays. Analyte capture agents can include antibodies, lectins, carbohydrates, polynucleotides, receptor proteins, and the like.
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Fu Elain
Hasenbank Melissa
Nelson Kjell
Yager Paul
Chin Christopher L
Townsend and Townsend and Crew
University of Washington UW Tech Transfer - Invention Licensing
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