Sigma binding region of RNA polymerase and uses thereof

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S252800, C435S325000

Reexamination Certificate

active

06905866

ABSTRACT:
The invention provides cells expressing the sigma binding region of the β′ subunit of RNA polymerase and a method to identify inhibitors of the formation of holoenzyme from core RNA polymerase and sigma.

REFERENCES:
patent: 5824546 (1998-10-01), Bishai et al.
patent: 6004764 (1999-12-01), Bishai et al.
patent: 6355469 (2002-03-01), Lam
patent: 2180077 (1997-01-01), None
patent: 2289862 (1996-09-01), None
patent: 2298862 (1996-09-01), None
patent: WO-99/64866 (1999-12-01), None
Arthur, T..M. ,et al. ,“Localization of a o70 binding site on the N terminus of theEscherichia coliRNA Polymeraase B' Subunit”,J. of Biol. Chem., vol. 273, No. 47, (Nov. 20, 1998),31381-31387.
Arthur, T..M. ,et al. ,“Mutational Analysis of Beta 260-309, a o70 Binding Site Located onEscherichia coliCore RNA Polymerase”,J. Biol. Chem., 275, (2000),23113.
Burgess, Richard.R. ,et al. ,“How sigma docks to RNA polymerase and what sigma does”,Current Opinion in Microbiology, (Mar., 2001),11 pages.
Burgess, R..R. ,et al. ,“Interaction ofEscherichia culio70 with Core RNA Polymerase”,Cold Spring Harbor Symposium, Cold Spring Harbor Press, NY, 63, (1998),277.
Burgess, R..R. ,et al. ,“Mapping Protein—Protein Interaction Domains Using Ordered Fragment Ladder Far-Western Analysis of Hexahistidine-Tagged Fusion Proteins”,Methods Enzymol., 328, (2000),141.
Burgess, R..R. ,“Purification of OverproducedEscherichia coliRNA Polymerase o Factors by Solubilizing Inclusion Bodies and Refolding from Sarkosyl”,Methods Enzymol., (1996),145.
Dearruda, M..,et al. ,“pET-33b(+): A pET Vector that Contains a Protein Kinase A Recognition Sequence”,InNovations(Novagen Newsletters), 4a, (1996),7.
Harpur, Ailsa,G. ,et al. ,“Imaging FRET between spectrally similar GFP molecules in single cells”,Nat. Biotechnol., 19, (2000),167.
Heyduk, E..,et al. ,“Architecture of a Complex between the o70 Subunit ofEscherichia coliRNA Polymerase and the Nontemplate Strand Oligonucleotide”,J. Biol. Chem., 274, (1999),3315.
Owens, J..T. ,et al. ,“Mapping the o70 subunit contact sites onEscherichia coliRNA polymerase with a o70-conjugated chemical protease”,Proc. Natl. Acad. Sci. U.S.A., 95, (1998),6021.
Pollok, Brian.A. ,et al. ,“Using GFP in FRET-based applications”,Trends Cell Biol., 9, (1999),57.
Rao, Lin.,et al. ,“Epitope Mapping Using Histidine-Tagged Protein Fragments: Application toEscherichia ColiRNA Polymerase”,Anal Biochem241, 173 (1996), 173-179.
Severinov, K..,et al. ,“Assembly of functionalEscherichia coliRNA polymerase containing Beta subunit fragments”,Proc. Natl. Acad. Sci. U.S.A., 92, (1995),4591.
Severinov, K..,et al. ,“Dissection of the Beta Subunit in theEscherichia coliRNA Polymerase into Domains by Proteolytic Cleavage”,J. Biol. Chem., 267, (1992),12813.
Severinov, K..,et al. ,“Structural Modules of the Large Subunits of RNA Polymerase”,J. Biol. Chem., 271, (1996),27969.
Severinov, K..,et al. ,“Tethering of the Large Subunits ofEscherichia coliRNA Polymerase”,J. Biol. Chem., 272, (1997),24137.
Sharp, M..M. ,et al. ,“The Interface of o with core RNA polymerase is extensive, conserved, and functionally specialized”,Genes and Development, vol. 13, (1999),3015-3026.
Thompson, N..E. ,et al. ,“Isolation and Characterization of Polyol-Responsive Monoclonal Antibody Useful for Gentle Purification ofEscherichia coliRNA Polymerase”,Biochemistry, 31, (1992),7003.
Wang, Daguang.,et al. ,“Discontinuous Movements of DNA and RNA in RNA Polymerase Accompany Formation of a Paused Transcription Complex”,Cell, 81, 341, 341-350.
Weilbaecher, Rodney.,et al. ,“Termination-altering amino acid substitutions in the B'subunit ofEscherichia coliRNA polymerase identify regions involved in RNA chain elongation”,Genes&Dev.8, 2913 (1994), 2913-2927.
Burgess, Richard , “Invited Talk”,Presented at the FASEB Meeting in Vermont, (Jul. 5, 1999),7 pages.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Sigma binding region of RNA polymerase and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sigma binding region of RNA polymerase and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sigma binding region of RNA polymerase and uses thereof will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3499516

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.