Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1997-10-24
2000-01-25
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
536 232, 4353201, 4352523, C12N 1554, C12N 910, C12N 1563
Patent
active
060177438
ABSTRACT:
The present invention provides a nucleotide sequence encoding Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransferase and an enzymatically active fragment thereof. The present invention also provides a nucleotide sequence encoding an extracellularly releasable protein capable of catalyzing Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransfer which comprises an enzymatically active fragment of the Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransferase together with a nucleotide sequence encoding at least one signal peptide. The present invention also provides recombinant vectors comprising nucleotide sequences encoding Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransferase. Processes for preparing extracellularly releasable polypeptide fragments of Sia.alpha.2,3Gal.beta.1,4GlcNAc .alpha.2,8-sialyltransferase are provided as well.
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Hamamoto Toshiro
Kojima Naoya
Kurosawa Nobuyuki
Tsuji Shuichi
Yoshida Yukiko
Prouty Rebecca E.
The Institute of Physical and Chemical Research
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