Shuttle vector, utilizing the E. coli asparagine synthetase gene

Details Shuttle vector, utilizing the E. coli asparagine synthetase gene Shuttle vector, utilizing the E. coli asparagine synthetase gene

1988-02-11

1991-04-30

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, 435 701, 435 91, 4352402, 935 34, 935 55, 935 70, 935 60, 935 79, C12N 1564, C12N 1585, C12N 1569, C12N 510

Patent

active

050117769

ABSTRACT:
A shuttle vector capable of dominant transfection of both animal and bacterial cells and of amplification in animal cells has been constructed. This vector contains the cloned bacterial gene coding for asparagine synthetase; it is shown that animal cells bearing the vector can be selected for with a drug which inhibits the animal but not the bacterial asparagine synthetase. The vector and any linked genes can then be amplified using a drug which inhibits the bacterial synthetase. This vector is useful in the production of large quantities of specific biological products by animal cells. It may also be useful for the genetic transformation of plants.

REFERENCES:
patent: 4656134 (1987-04-01), Ringold
Nakamura et al., Nucleic Acids Research vol. 19 #18 1981 pp. 4669-4676.
Cartier et al., Mol. and Cell. Biol. vol. 7 #5 (1987) pp. 1623-1628.
Chemical Abstracts, 98:192668s [(1983), Waye et al., J. Mol. Appl. Genet. 1), 69-82 (1983)].

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