Shuttle vector comprising a T-DNA Region and RI and RK2 origins

Details Shuttle vector comprising a T-DNA Region and RI and RK2 origins Shuttle vector comprising a T-DNA Region and RI and RK2 origins

1997-09-25

2000-12-26

McKelvey, Terry

Chemistry: molecular biology and microbiology

Vector, per se

C12N 1570, C12N 1574

Patent

active

061657808

ABSTRACT:
The invention provides a high capacity binary shuttle vector having T-DNA region and Ri ori and capable to integrate a large genome fragment in it; a genomic library having the ability to transform a plant, especially monocotyledonous ones; a plant transformed with the high capacity binary shuttle vector; and a method of searching for a useful gene by use of the above vector. The present high capacity binary shuttle vector can introduce a large genome fragment of 10 kb or more, easily, efficiently and stably into a plant, especially monocotyledonous ones under those conditions in which the rearrangement or deletion of these genome fragments does not occur. The invention also provides some other vectors derived from the above Ri ori driven vectors, which can integrate circular genome library plasmids with a lox site, or which can efficiently transform plants with genes of arbitrary expression properties. The invention includes also the assessment method for the functions of genome fragments inserted in the above vectors by complementation assay. In addition, a useful gene or gene complex can be efficiently examined so its isolation is also made easy.

REFERENCES:
patent: 5614395 (1997-03-01), Ryals et al.
Molecular Analysis of the RecA gene of Agrobacterium tumefaciens C58, Wardhan, et al., Gene 121 (1992) 133-136. Interspecies regulation of the recA gene of gram-negative bacteria lacking an E. coli-like SOS operator, Riera et al., Mol. Gen. Genet (1994) 245:523-527.

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