Shuttle vector

Chemistry: molecular biology and microbiology – Vector – per se

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C12N 1500, C12N 1509

Patent

active

057078629

ABSTRACT:
A shuttle vector containing an yeast gene and an E. coli gene and carrying the expression control region of acid phosphatase gene of yeast, which can be recombined with various genes under control of the phosphatase promoter, to give various recombinant plasmids. The shuttle vector is useful in genetic engineering industries.

REFERENCES:
patent: 4387162 (1983-06-01), Aigle et al.
patent: 4778761 (1988-10-01), Miyanohara et al.
patent: 4997767 (1991-03-01), Nozaki et al.
R.A. Hitzeman, et al., Expression of a human gene for interferon in yeast, Nature, vol. 293, pp. 717-722, (1981).
B. Meyhack, et al., Two yeast acid phosphatase structural genes are the result of a tandem duplication and show different degrees of homology in their promoter and coding sequences, The Embo Journal, vol. 1, No. 6, pp. 675-680, (1982).
J. Ferguson, et al., Construction and characterization of three yeast Escherichia coli shuttle vectors designed for rapid subcloning of yeast genes on small DNA fragments, Gene, 16, pp. 191-197, (1981).
H. Rudolph, et al., The yeast PHO5 promoter: Phosphate-control elements and sequences mediating mRNA start-site selection, Proc. Natl. Acad. Sci., USA, vol. 84: 1340-44 (1987).
P. Valenzuela et al., Nature, vol. 298, pp. 347-350, (1982).
K. Arima et al., The nucleotide sequence of the yeast PHO5 gene: a putative precursor of repressible acid phosphatase contains a signal peptide, Nucleic Acids Research vol. 11(6): 1657-72 (1983).
Struhl (1981), Proc. Nat. Acad. Sci, vol. 78, pp. 4461-4465.

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