Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2007-11-13
2007-11-13
Robinson, Hope (Department: 1652)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C530S300000, C514S012200
Reexamination Certificate
active
10380734
ABSTRACT:
In this invention, three genes encoding short chain neurotoxin are obtained by sea snake—Lapemis hardwickiivenom gland cDNA library construction and DNA sequencing, named sn12, sn36, and sn160, respectively. The three genes are modified and amplified by PCR method, then cloned into expression vector PETTRX. The three neurotoxin genes are all highly expressed in soluble fusion protein inE. colistain BL21-DE3 which is used as a expression host when induced with IPTG. Studies on the analgesic effects of the three recombinant short chain neurotoxins showed that three proteins, SN12, SN36 and SN160 all can significantly increase the pain threshold value of mouse.
REFERENCES:
patent: WO 98/42834 (1998-10-01), None
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“Amino Acid Sequence of a Snake Neurotoxin from the Venom ofLapemis hardwickiiand the Detection of a Sulphydryl Group of Laser Raman Spectroscopy”, Jay W. Fox et al., Febs Letters, vol. 80 (1), pp. 217-220 (1977).
“Amino Acid Sequence of Pelamitoxin a, The Main Neurotoxin of the Sea Snake,Pelamis platurus”, C.L. Wang et al., Toxicon vol. 14, pp. 456-466 (1976).
“Hydrophitoxin b fromHydrophis cyanocinctusVenom”, C.S. Liu et al., Toxicon vol. 12, pp. 543-546 (1974).
Peng Lisheng
Wei Jianwen
Wu Wenyan
Xu Anlong
Yang Wenli
Robinson Hope
Sawicki Peter
Zhongshan University
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