Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1995-09-06
1998-10-20
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
424 932, 4351721, 4351723, 435245, 4352523, 435822, C12N 100, C12N 120, C12N 1500
Patent
active
058245383
ABSTRACT:
We describe a bacterial delivery system for the delivery of DNA and antigens into cells. We constructed an attenuated bacterial vector which enters mammalian cells and ruptures delivering functional plasmid DNA, such as a mammalian expression plasmid, and antigens into the cell cytoplasm. This Shigella vector was designed to deliver DNA to colonic surfaces, thus opening the possibility of oral and other mucosal DNA immunization and gene therapy strategies. The attenuated Shigella is also useful as a vaccine for reducing disease symptoms caused by Shigella.
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Branstrom Arthur A.
Sadoff Jerald C.
Sizemore Donata R.
Harris Charles H.
Lankford , Jr. Leon B.
Moran John Francis
Tate Christopher R.
The United States of America as represented by the Secretary of
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