Shielded stationary phases

Details Shielded stationary phases Shielded stationary phases

1992-12-10

1994-01-11

Therkorn, Ernest G.

Liquid purification or separation

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Material

210635, 210656, 2101982, 502401, B01D 1508

Patent

active

052778134

ABSTRACT:
Novel packing materials are provided for liquid chromatography and/or solid phase extraction columns which will allow direct injection of biological fluids. These packing materials have a hydrophilic exterior layer and a hydrophobic, charged or otherwise selective portion that forms an underlayer or is embedded in the hydrophilic layer. During a chromatographic process large water soluble biopolymers will be in contact with the hydrophilic outer layer and be shielded from interacting with the underlayer or embedded portion and elute unretained. Small analytes, on the other hand, can be fully partitioned throughout the exterior and interior layers and are retained by hydrophobic or electrostatic interactions. Using such packings the direct analyses of plasma or serum for drug analysis is demonstrated.

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