Shelf-stable product and process for isolating RNA, DNA and prot

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

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536 254, 536 2541, 935 19, 935 20, C07H 2102

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active

053469941

ABSTRACT:
Shelf-stable solvent solutions and methods for simultaneously isolating RNA, DNA and proteins from biological samples are disclosed. The solvent solutions include phenol and a guanidinium compound, preferably at a concentration below about 2M, which is effective in isolating substantially pure and undegraded RNA, substantially pure and undegraded DNA, and proteins from the same biological sample.

REFERENCES:
patent: 4843155 (1989-06-01), Chomczynski
patent: 5010183 (1991-04-01), Macfarlane
patent: 5128247 (1992-07-01), Koller
patent: 5130423 (1992-07-01), Van Ness et al.
patent: 5234809 (1993-08-01), Boom et al.
Coombs, L. M., Pigott, D., Proctor, A., Eydmann, M., Denner, J. and Knowles, M. A. "Simultaneous Isolation of DNA, RNA, and Antigenic Protein Exhibiting Kinase Activity from Small Tumor Samples Using Guanidine Isothiocyanate", Anal. Biochem. 188, 338-343 (1990).
Sela, M., Anfinsen, C. B. & Harrington, W. F. "The Correlation of Ribonuclease Activity With Specific Aspects of Tertiary Structure", Biochem. Biophys. Acta 26, 506-511 (1957).
Cox, R. A. "The Use of Guanidinium Chloride in the Isolation of Nucleic Acids", Methods in Enzymology 12(B):120-129 (1968).
Nicolaides, N. C. & Stoeckert, Jr., C. J. "A Simple, Efficient Method for the Separate Isolation of RNA and DNA from the Same Cells", Biotechniques 8, 154-156 (1990).
Raha, S., Merante, F., Proteau, G. and Reed, J. K. "Simultaneous Isolation of Total Cellular RNA and DNA from Tissue Culture Cells Using Phenol and Lithium Chloride", Gene Anal. Techn. 7, 173-177 (1990).
Chan, V. T. W., Fleming, K. A. & McGee, J. O'D. "Simultaneous Extraction from Clinical Biopsies of High-Molecular-Weight DNA and RNA: Comparative Characterization by Biotinylated and .sup.32 P-Labeled Probes on Southern and Northern Blots", Anal. Biochem. 168, 16-24 (1988).
Attardi, B. & Miklos, J. "Rapid Stimulatory Effect of Activin-A on Messenger RNA Encoding the Follicle-Stimulating Hormone Beta-Subunit in Rat Pituitary Cell Cultures", Molec. Endocrin. 4, 721-726 (1990).
Attardi, B. & Fitzgerald, T. "Effects of Progesterone on the Estradiol-Induced Follicle-Stimulating Hormone (FSH) Surge and FSH Beta Messenger Ribonucleic Acid in the Rat", Endocrinology 126, 2281-2287 (1990).

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