Sexing method of bovine embyros

Details Sexing method of bovine embyros Sexing method of bovine embyros

1992-12-02

1995-10-24

Parr, Margaret

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 2433, 435 6, 435 912, C07H 2104, C12P 1934, C12Q 168

Patent

active

054611458

ABSTRACT:
The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.

REFERENCES:
patent: 4960690 (1990-10-01), Ellis et al.
patent: 5055393 (1991-10-01), Kwoh et al.
Ellis et al., Theriogenology, vol. 29, No. 1, "Sex Determination of Bovine Embryos Using Male-Specific DNA Probes," Jan., 1988, p. 242.
European Search Report, Mar. 19, 1993.

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