Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-06-02
1997-08-26
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
536 2433, 536 2431, C12P 1934
Patent
active
056610114
ABSTRACT:
The present invention provides PCR primers with which sexing of bovine embryos can be easily attained and provides a practical, rapid and reliable method for determining the sex of bovine embryos using these primers. The methods for determining the sex of the bovine embryos are characterized by discriminating PCR products which are obtained by amplifying specific DNA sequences by PCR with pairs of male-specific and gender-neutral primers. These primers are derived from DNAs which specifically hybridize to the bovine male genome and from DNAs which gender-neutrally hybridize to both bovine male and female genomes.
REFERENCES:
patent: 4960690 (1990-10-01), Ellis et al.
patent: 5055393 (1991-10-01), Kwoh et al.
Anderson et al., 1982, "Complete Sequence of Bovine Mitochondrial DNA, Conserved Features of the Mammalian Mitochondrial Genome," J. Mol. Biol. 156:683-717.
Ellis et al., 1988, "Sex Determination of Bovine Embryos Using Male-Specific DNA Probes," Theriogenology 29(1):242.
European Search Report, Mar. 19, 1993.
Anderson et al., 1982,, "Complete Sequence of Bovine Mitochondrial DNA, Conserved Features of the Mammalian Mitochondrial Genome," J. Mol. Biol. 156:683-717.
Bondioli et al. (1989) "The Use of Male-Specific Chromosomal DNA Fragments to Determine the Sex of Bovine Preimplantation Embryos", Theriogenology vol. 31:95-104.
Itagaki Yoshiaki
Kudo Toshiyuki
Nakamura Toyoo
Sato Seiji
Sutou Shizuyo
Horlick Kenneth R.
Itoham Foods Inc.
Tung Joyce
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