Sesquiterpene synthases from patchouli

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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Details

C435S183000, C435S320100, C435S252300, C435S325000, C435S410000, C536S023100, C536S023200, C536S023600

Reexamination Certificate

active

07622288

ABSTRACT:
The invention relates to sesquiterpene synthases from Patchouli plants (Pogostemon cablin), and methods of their production and use. In one embodiment, the invention provides nucleic acids comprising a nucleotide sequence as described herein that encodes for at least one sesquiterpene synthase. In a further embodiment, the invention also provides for sesquiterpene synthases and methods of making and using these enzymes. For example, sesquiterpene synthases of the invention may be used to convert farnesyl-pyrophosphate to various sesquiterpenes including patchoulol, γ-curcumene and other germacrane-type sesquiterpenes.

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C. L. Steel et al., XP002279809, “Sesquiterpene Synthases From Grand Fir (Abies grandis). Comparison Of Constitutive And Wound-Induced Activities, And cDNA Isolation, Characterization, And Bacterial Expression Of Delta-Selinene Synthase and Gamma-Humulene Synthase” Journal Of Biological Chemistry, vol. 273, No. 4, pp. 2078-2089 (1998).

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