Serum with reduced levels of steroids

Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Blood serum or blood plasma standard or control

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436 17, 436 18, 436817, G01N 3378, G01N 3396

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active

053087682

ABSTRACT:
A blood serum sample useful as a calibration baseline comprising a natural blood matrix is disclosed. This serum sample contains less than an HPLC-detectable amount of cortisol. Also disclosed is a serum sample containing less than HPLC-detectable amounts of cortisone and corticosterone and less than RIA-detectable amounts of testosterone, androstenedione, progesterone, DHEA sulfate and 17-OH progesterone. A method of preparing such a serum sample is also disclosed.

REFERENCES:
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patent: 4784960 (1988-11-01), Baranczuk
Textbook of Medicine, 19th Ed. Cecil, Ed. Wyngaard, Smith, Bennett, copyright 1992, pp. 1271-1272.
"Evaluation of SeraSub.TM.. . . For Making Calibrators and Controls for Drugs of Abuse", Clin. Chem. 37(6) 1991, Akerka.
"Evaluation of SeraSub.TM. a substitute . . . for making calibrators and controls", Alan P. Schwartz, Clin Chem 36(6) 1025, Jul. 1990.
Poster presented Jul., 1992 "Determination of cortisol (c), cortisone (cn), corticosterone (cc), prednisone (p), and prednisolone (pl) in serum with solid reversed-phase extraction and high performance liquid chromatography (HPLC)". Gary L. Lensmeyer, et al., Clin. Labs., (Univ. Wisc. Hosp. & Clinics, Dept. Path. & Lab. Med., Madison, Wis.).
Frey, et al., "Liquid-Chromatography Measurement of Endogenous and Exogenous Glucocorticoids in Plasma", Clinical Chemistry, vol. 25, No. 11:1944-1947, 1979.
McBride, et al., "Rapid Liquid-Chromatographic Method for Simultaneous Determination of Plasma Prednisone, Prednisolone, and Cortisol in Pediatric Renal-Transplant Recipients", Clinical Chemistry, vol. 37, No. 5:643-646, 1991.
Soldin, et al., "Development of a Radioreceptor Assay to Measure Glucocorticoids", Therapeutic Drug Monitoring, 14:164-168, 1992.
William Vine, Letters to the Editor, "Comments on Proficiency Testing", Clinical Chemistry, vol. 38, No. 10, 1992.
Advertisement from Biomedical Products, Jul., 1992 "Scantibodies Laboratory, Steroid Free Human Serum in Two Forms".
Advertisement from Creative Scientific Technology, Inc., Jun., 1993, "SeraSub --Liquid, Protein-Free Serum Substitute For Use in Manufacturing Clinical Chemistry and Immunological Standards and Controls".

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