Serum vitamin B.sub.12 assay and kit therefor

Details Serum vitamin B.sub.12 assay and kit therefor Serum vitamin B.sub.12 assay and kit therefor

1980-02-04

1981-11-17

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

232303, 422 61, 424 1, G01N 3382, G01N 3358

Patent

active

043009077

ABSTRACT:
In a vitamin B.sub.12 assay, serum, containing vitamin B.sub.12 bound to endogenous binders therefor, is released from such endogenous binders by a releasing agent comprised of a water miscible organic liquid, such as, acetone, reducing agent and cyanide ions. Such release can be effected at room temperature, as compared to prior procedures which required heating to elevated temperatures.

REFERENCES:
patent: 3937799 (1976-02-01), Lewin
patent: 3980764 (1976-09-01), Adams
patent: 4028465 (1977-06-01), Lewin
patent: 4167556 (1979-09-01), Selhub
patent: 4188189 (1980-02-01), Allen
J. I. Toohey, et al., J. Biol. Chem., 236(2), 560-563 (1961).
W. A. Fenton, et al., Anal Biochem., 90(1), 119-125 (1978).

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