Serum pretreatment for digoxin assay

Details Serum pretreatment for digoxin assay Serum pretreatment for digoxin assay

1984-06-15

1987-03-31

Turk, Arnold

Chemistry: analytical and immunological testing

Including sample preparation

Digestion or removing interfering materials

210656, 436 94, 436161, 436178, 436825, G01N 3002, G01N 3350

Patent

active

046543112

ABSTRACT:
Serum is passed through a column containing silica gel alkylated with lower alkyl groups. The column is then washed with diluted acid and water, and the digoxin eluted with aqueous methanol at a volume less than about the volume of the serum to provide a digoxin concentrate that may be used in assay determinations.

REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 3981982 (1976-09-01), Oslapas et al.
Henry, "Clinical Diagnosis and Management by Laboratory Methods", 17th ed., Saunders & Co., 1984, pp. 349-351.
Davidsohn et al., "Clinical Diagnosis by Laboratory Methods", 15th ed., Saunders & Co., 1974, pp. 677-678.
Lindner et al., Journal of Chromatography, 117(1976) 81-86.
Fujii et al., Journal of Chromatography, 202 (1980) 139-143.
Davydov et al., Journal of Chromatography, 204 (1981) 293-301.
Davydov et al., Journal of Chromatography, 248 (1982) 49-62.
Pekic et al., Journal of Chromatography, 268 (1983) 237-244.
Kabra et al., "Clinical Liquid Chromatography", vol. I-Analysis of Exogenous Compounds CRC Press, 1984, 197-208.

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