Serum-free, synthetic, completely chemically defined tissue cult

Details Serum-free, synthetic, completely chemically defined tissue cult Serum-free, synthetic, completely chemically defined tissue cult

1984-09-13

1987-04-14

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435241, 435948, C12N 500, C12N 502, C12R 191

Patent

active

046578668

ABSTRACT:
A serum-free, synthetic tissue culture media is described which is completely defined chemically. The two media described can be used for growing all types of human or animal cell lines in tissue culture without addition of any protein, amino acids, hormones, sources of energy, salts, vitamins, etc. with normally used procedures and methods. The media do not require any supplementation with fetal calf serum to support growth of neural cells.

REFERENCES:
Honegger et al., "Growth and Differentiation of Aggregating Fetal Brain Cells in a Serum-Free Defined Medium" Nature 282 pp. 305-308 (1979).
Monte, "Hormone-Dependant Growth and Differentiation of Brain Cultured in Serum-Free Medium" Federation Proceedings 38(3) p. 1396 (1979) Abst. #6163.
Del'Aquila et al., "Growth of Normal Human Amnion Epithelial Cells in Serum-Free Medium" Experimental Cellular Research 137 pp. 441-446 (1982).

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