Serum-free medium supplement

Details Serum-free medium supplement Serum-free medium supplement

1995-03-24

1996-04-30

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352403, C12N 502

Patent

active

055124771

ABSTRACT:
This invention relates to a serum-free eukaryotic cell culture medium supplement. The supplement comprises carbon sources, vitamins, inorganic salts, amino acids and a protein digest. The medium supplement of the present invention enables the maintenance of mammalian cell cultures at cell densities equal to or greater than that obtained with batch culture methods while increasing longevity and productivity.

REFERENCES:
patent: 4282326 (1981-08-01), Moldenhauer
patent: 5122469 (1992-06-01), Mather et al.
Sigma Cell Culture Reagents Catalogue, 1992, pp. 232, 262 and 263.
Jo, E. et al. (1993) "Repeated Fed-Batch Culture of Hybridoma Cells in Nutrient-Fortified High-Density Medium" Biotechnology And Bioengineering, 42(10):1228-1237.
Fike, R. et al (1993) "Feeding Strategies for Enhanced Hybridoma Productivity: Automated Concentrate Supplementation" BioPharm, Oct.:49-54.
Luan, Y. T. et al. (1987) "Strategies to Extend Longevity of Hybridomas in Culture and Promote Yield of Monoclonal Antibodies" Biotechnology Letters, 9(10):691-696.
Reuveny, S. et al. (1985) "Production of Monoclonal Antibodies in Culture" Develop. biol. Standard, 60:185-197.

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