Serum-free medium for cultivation of postnatal central neurons

Details Serum-free medium for cultivation of postnatal central neurons Serum-free medium for cultivation of postnatal central neurons

1995-02-13

1998-07-14

Saucier, Sandy

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Culture medium, per se

435405, 435406, 435407, 435381, 435386, 435387, 435388, 435394, C12N 500, C12N 502

Patent

active

057803013

ABSTRACT:
Disclosed is a synthetic medium with PDGF, vitronectin, IL-1.beta. and BSA added to Eagle's minimum essential medium or medium with transferrin, insulin, progesterone and putrescine further added thereto. When cultivating the postnatal central neurons using the inventive medium, there are effects such that good attachment to substrate, extension of neuritic processes and maintenance of survival are achieved, that more stable sure cultivation becomes possible as well over the astrocyte-conditioned medium used hitherto, and the like.

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