Serum-free growth medium and use thereof

Details Serum-free growth medium and use thereof Serum-free growth medium and use thereof

1990-02-23

1991-09-03

Stone, Jacqueline

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

43524031, C12Q 102, C12N 502

Patent

active

050454544

ABSTRACT:
The present invention discloses a serum-free growth medium with iron-enhancing cell culture characteristics provided by a transferrin-replacing substance. The transferrin replacement preferably comprises an iron-chelate, aurintricarboxylic acid and alkali-metal-EDTA. More particularly, the iron-chelate comprises a mixture of citrate/citric acid and Fe/EDTA. Trace elements and growth factors are also included in the disclosed medium.
The growth medium further comprises base culture media. Most preferably, the base media comprise a mixture of RPMI 1640, HEPES and NaHCO.sub.3. The growth medium together with a culture of hybridoma eukaryotic cells may be used for quality testing of other growth media, optionally together with a preferred hybridoma cell line from P.sub.3 U.sub.1 tumor cells and B-lymphocytes. This test procedure is provided by way of a containerized kit which includes the serum-free medium and the most preferred fast-growing eukaryotic cell line, hybridoma ECACC 86 112001.

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