Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...
Reexamination Certificate
2006-02-28
2006-02-28
Woitach, Joseph (Department: 1632)
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
C435S325000, C435S326000, C435S004000, C435S041000, C800S008000
Reexamination Certificate
active
07005252
ABSTRACT:
Disclosed herein are methods for culturing primate embryonic stem cells. These cells are cultured on a prolonged and stable basis in the presence of exogenously supplied fibroblast growth factor and in the absence of animal serum. Preferably there is also a fibroblast feeder layer. Also disclosed is a culture media containing fibroblast feeder layer and the fibroblast growth factor.
REFERENCES:
patent: 5405772 (1995-04-01), Ponting
patent: 5453357 (1995-09-01), Hogan
patent: 5670372 (1997-09-01), Hogan
patent: 5690926 (1997-11-01), Hogan
patent: 5843780 (1998-12-01), Thomson
patent: 6200806 (2001-03-01), Thomson
patent: 6245566 (2001-06-01), Gearhart et al.
patent: 2002/0081724 (2002-06-01), Carpenter et al.
patent: 19756864 (1997-12-01), None
patent: 97/47734 (1997-12-01), None
patent: 98/30679 (1998-07-01), None
patent: 99/20741 (1999-04-01), None
patent: 00/68359 (2000-11-01), None
JA Thomson et al., Proc.Natl.Acad.Sci USA, “Isolation of a primate embryonic stem cell line,” Aug. 1995, vol. 92, pp. 7844-7848.
MD Goldsborough et al., Focus, “Serum-Free Culture of Murine Embryonic Stem (ES) Cells,”1998, vol. 20, No. 1,pp. 8-12.
AJ Clark et al., Transgenic Animals, “Germ line manipulation: applications in agriculture and biotechnology,” Chap. 11, p. 250.
J Nichols et al., Development,“Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity,” 1990, 110, pp. 1341-1348.
JA Piedrahita et al., Theriogenology,“ On the Isolation of Embryonic Stem Cells: Comparative Behavior of Murine, Procine and Ovine Embryos,” Nov. 1990, vol. 34, No. 5, pp. 879-901.
YP Cruz et al., Animal Applications of Research in Mammalian Deveolpment,“Origin of Embryonic and Extraembryonic Cell Lineages in Mammalian Embryos,” pp. 147-204.
J. Thomson et al., Embryonic Stem Cell Llnes Drived From Human Blastocysts, 282 Science 1145-1147 (1998).
J. Rossant et al., In Search Of The tabula rasa Of Human Cells, 17 Nature Biotechnology 23-4 (1999).
J. Gearhart, New Potential For Human Embryonic Stem Cells, 282 Science 1061-2 (1998).
T. Yamaguchi et al., Expression Of The Fibroblast Growth Factor Receptor FGFR-1/flg During Gastrulation And Segmentation In the Mouse Embryo, 152 Dev. Biol. 75-88 (1992).
Mummery et al., “Fibroblast Growth Factor-Mediated Growth Regulation and Receptor Expression in Embryonal Carcinoma and Embryonic Stem Cells and Human Germ Cell Tumours,”Biochemical and Biophysical Research Communications191:188-195 (1993).
Pease et al., “Isolation of Embryonic Stem (ES) Cells in Media Supplemented with Recombinant Leukemia Inhibitory Factor (LIF),”Developmental Biology141:344-352 (1990).
Quarles & Brady LLP
Wisconsin Alumni Research Foundation
Woitach Joseph
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