Serum-free cell culture medium and process for making same

Details Serum-free cell culture medium and process for making same Serum-free cell culture medium and process for making same

1982-12-16

1985-12-24

Rosen, Sam

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435240, C12N 502, C12N 500

Patent

active

045606552

ABSTRACT:
A defined serum-free medium that is capable of growing a wide range of suspension and monolayer cells includes a serum substitute composed of fetuin, transferrin, phosphatidylcholine, e.g., 1-oleoyl-2-palmitoyl-phosphatidylcholine, linoleic acid and cholesterol. The medium also includes various inorganic salts, carbohydrates, amino acids, buffering agents, vitamins, and compounds to simulate the natural cell environment.

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