Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1985-09-09
1989-03-28
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352403, 4352402, 4352401, C12N 500
Patent
active
048164011
ABSTRACT:
A serum free cell culture medium containing less than 10 ug/ml protein is disclosed. The medium comprises a basal media supplemented with levels of 2-aminoethanol substantially higher than previously disclosed and supplemented with 2-mercaptoethanol, transferrin, insulin and free essential amino acids. Unlike other serum free media, the media disclosed herein will support the growth and long term culture of a large variety of cell types including hybridomas and tumor cell lines while maintaining both growth potential and the differentiated characteristics of the particular cell line.
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Lord Edith M.
Taupier Mary A.
Lukacher Martin
Patterson, Jr.
University of Rochester
Wiseman Thomas G.
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