Serum-free and mitogen-free T-cell growth factor and process for

Details Serum-free and mitogen-free T-cell growth factor and process for Serum-free and mitogen-free T-cell growth factor and process for

1981-03-26

1983-06-28

Rosen, Sam

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435948, 435240, 435284, C12P 2100

Patent

active

043906230

ABSTRACT:
A serum-free and mitogen-free T-cell growth factor (interleukin-2) preparation is prepared from human, bovine, or procine peripheral mononuclear blood cells which are washed several times with a liquid tissue culture medium and then stimulated in a tissue culture medium supplemented with serum and mitogen. The separated stimulated cells are again washed with fresh tissue culture medium to remove substantially all of the serum and mitogen. The washed cells are suspended in fresh tissue culture medium and conditioned under incubation conditions to transfer the growth factor into the liquid. The tissue culture medium separated from the stimulated cells can be recycled to stimulate additional cells. The supernatant can be concentrated from 50 to 100-fold on an ultrafilter. The supernatant can activate the production of natural killer cells in patients suffering from tumors.

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