Serum fluorescence suppression

Details Serum fluorescence suppression Serum fluorescence suppression

1977-03-02

1978-07-11

Smith, Alfred E.

Radiant energy

Luminophor irradiation

With ultraviolet source

356 39, 356 85, G01N 2138, G01N 3316, G01J 330

Patent

active

041004167

ABSTRACT:
A system for suppressing normal serum fluorescence in fluorescence immunoassay, in which in one embodiment, the immunoassay fluorescently tagged reaction product in a single sample is irradiated at a first wavelength which will excite fluorescence from the tagged product and then at a different wavelength which is not substantially in an absorption band of the fluorescent tag, the respective fluorescent emissions being detected and converted to electrical signals. The difference between the signals, adjusted for variation in serum fluorescence at the two wavelengths, is a function of the antigen titer. In an alternative form, the sample is irradiated at the first wavelength for a predetermined period of time commencing very shortly after the emission reaction is started, and the resulting signal from the detector is differentiated. In both instances, output signal due to serum fluorescence per se is suppressed.

REFERENCES:
patent: 3433952 (1969-03-01), Howerton
patent: 3918812 (1975-11-01), Holm
patent: 3973129 (1976-08-01), Blumberg et al.

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