Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2005-02-15
2005-02-15
Ketter, James (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S006120, C536S022100
Reexamination Certificate
active
06855521
ABSTRACT:
Five fluorogenic probe hydrolysis reverse transcriptase-polymerase chain reaction (RT-PCR) (TaqMan™) assays are described for serotype-specific detection of dengue 1-4 and group-specific detection of dengue viruses. Type- and group-specific oligonucleotide primers and fluorogenic probes were designed against conserved regions of the dengue genome. The invention provides TaqMan PCR assays, which are rapid, sensitive, and specific screening and serotyping tools for the epidemiological study of dengue infections.
REFERENCES:
Laue et al, Detection of Dengue virus RNA in patients after primary or secondary dengue infection using the TaqMan automated amplification system, Journal of Clinical Microbiology, 1999, vol. 37(8), pp. 2543-2547.
Callahan Johnny Dale
Temenak Joseph John
Garvey Tara L.
Hemby, Jr. Joseph K.
Ketner Philip E.
Ketter James
The United States of America as represented by the Secretary of
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