Serine protease variants having amino acid deletions and...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S220000, C435S221000, C435S222000, C435S471000, C510S392000, C424S094640, C536S023200

Reexamination Certificate

active

06908757

ABSTRACT:
The present invention relates to variants of serine proteases having decreased immunogenicity relative to their corresponding wild-type proteases. More particularly, the present invention relates to variants having a modified amino acid sequence of a wild-type amino acid sequence, wherein the modified amino acid sequence comprises a deletion and, optionally, a substitution of one or more specifically identified positions corresponding to subtilisin BPN′. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.

REFERENCES:
patent: 4179337 (1979-12-01), Davis et al.
patent: 4248786 (1981-02-01), Batz et al.
patent: 4266031 (1981-05-01), Tang et al.
patent: 4556554 (1985-12-01), Calvo
patent: 4732863 (1988-03-01), Tomasi et al.
patent: 4760025 (1988-07-01), Estell et al.
patent: 4980288 (1990-12-01), Bryan et al.
patent: 5009813 (1991-04-01), Watanabe et al.
patent: 5122614 (1992-06-01), Zalipsky
patent: 5133968 (1992-07-01), Nakayama et al.
patent: 5208158 (1993-05-01), Bech et al.
patent: 5230891 (1993-07-01), Nakayama et al.
patent: 5324844 (1994-06-01), Zalipsky
patent: 5414135 (1995-05-01), Snow et al.
patent: 5446090 (1995-08-01), Harris
patent: 5543302 (1996-08-01), Boguslawski et al.
patent: 5545402 (1996-08-01), Watkinson
patent: 5567601 (1996-10-01), Bryan et al.
patent: 5631322 (1997-05-01), Veronese et al.
patent: 5658871 (1997-08-01), Batenburg et al.
patent: 5665366 (1997-09-01), Rawlings et al.
patent: 5766898 (1998-06-01), Loevburg
patent: 5856451 (1999-01-01), Olsen et al.
patent: 5972339 (1999-10-01), Walker
patent: 5985264 (1999-11-01), Metzger et al.
patent: 6060546 (2000-05-01), Powell et al.
patent: 2 206 826 (1973-08-01), None
patent: 0 130 756 (1985-01-01), None
patent: 0 215 662 (1987-03-01), None
patent: 0 260 105 (1988-03-01), None
patent: 0 398 539 (1990-11-01), None
patent: 0 405 901 (1991-01-01), None
patent: 0 471 125 (1992-12-01), None
patent: 0 516 200 (1992-12-01), None
patent: 0 584 876 (1994-03-01), None
patent: 0 251446 (1994-12-01), None
patent: 0 816 381 (1998-01-01), None
patent: WO 87/04461 (1987-07-01), None
patent: WO 87/05050 (1987-08-01), None
patent: WO 88/07578 (1988-10-01), None
patent: WO 88/08028 (1988-10-01), None
patent: WO 88/08033 (1988-10-01), None
patent: WO 88/08164 (1988-10-01), None
patent: WO 88/08165 (1988-10-01), None
patent: WO 91/00345 (1991-01-01), None
patent: WO 92/10755 (1992-06-01), None
patent: WO 93/15189 (1993-08-01), None
patent: WO 19732 (1993-10-01), None
patent: WO 93/19731 (1993-10-01), None
patent: WO 93/25667 (1993-12-01), None
patent: WO 94/04193 (1994-03-01), None
patent: WO 94/06905 (1994-03-01), None
patent: WO 95/07991 (1995-03-01), None
patent: WO 95/10615 (1995-04-01), None
patent: WO 95/20039 (1995-07-01), None
patent: WO 95/29979 (1995-11-01), None
patent: WO 95/30010 (1995-11-01), None
patent: WO 96/09396 (1996-03-01), None
patent: WO 96/09396 (1996-03-01), None
patent: WO 96/16177 (1996-05-01), None
patent: WO 96/17929 (1996-06-01), None
patent: WO 96/40791 (1996-12-01), None
patent: WO 96/40792 (1996-12-01), None
patent: WO 97/07770 (1997-03-01), None
patent: WO 97/24421 (1997-07-01), None
patent: WO 97/24427 (1997-07-01), None
patent: WO 97/30148 (1997-08-01), None
patent: WO 97/37007 (1997-10-01), None
patent: WO 98/23732 (1998-06-01), None
patent: WO 98/30682 (1998-07-01), None
patent: WO 98/35026 (1998-08-01), None
patent: WO 99/00489 (1999-01-01), None
patent: WO 99/33868 (1999-07-01), None
patent: WO 99/37324 (1999-07-01), None
patent: WO 99/40936 (1999-08-01), None
patent: WO 99/41369 (1999-08-01), None
patent: WO 99/42097 (1999-08-01), None
patent: WO 99/44635 (1999-09-01), None
patent: WO 99/45904 (1999-09-01), None
patent: WO 99/48918 (1999-09-01), None
patent: WO 99/49056 (1999-09-01), None
patent: WO 00/09707 (2000-02-01), None
patent: WO 00/28007 (2000-05-01), None
patent: WO 00/37658 (2000-06-01), None
Arlian, L.G. et al., “Antigenic and Allergenic Characteristics of the Enzymes Alcalase and Savinase by Crossed Immunoelectrophoresis and Crossed Radioimmunoelectrophoresis”, Int. Arch. Allergy. Appl. Immunol., vol. 91, pp. 278-284 (1990).
Abuchowski, A. et al., “Cancer Therapy with Chemically Modified Enzymes. I. Antitumor Properties of Polyethylene Glycol-Asparaginase Conjugates”, Cancer Biochem. Biophys., vol. 7, pp. 175-186 (1984).
Abuchowski, A. et al., “Soluble Polymer-Enzyme Adducts”, Rutgers University, New Brunswick, NJ, Chapter 13, pp. 367-383.
Bungy Poor Fard, G.A. et al., “T Cell Epitopes of the Major Fraction of Rye Grass Lolium Perenne (lol p 1) Defined Using Overlapping Peptides in Vitro and In Vivo. I. Isoallergen Clone 1A”, Clin. Exp. Immunol., vol. 94, pp. 111-116 (1993).
Caliceti, P. et al., “Active Site Protection of Proteolytic Enzymes by Poly(ethylene glycol) Surface Modification” Journal of Bioactive and Compatible Polymers, vol. 8, pp. 41-50 (Jan. 1993).
Cunningham, B. C. et al., “Improvement in the alkaline stability of subtilisin using an efficient Random Mutagenesis and Screening Procedure”, Protein Engineering, vol. 1, No. 4, pp. 319-325 (Aug./Sep. 1987).
Davis, F. F. et al., “Reduction of Immunogenicity and Extension of Circulating Half-Life of Peptides and Proteins”, Peptide and Protein Drug Delivery, Chpt. 21, pp. 831-857, Lee, V. (ED), University of California School of Pharmacy, Los Angeles, CA.
Delgado, C. et al., “The Uses and Properties of PEG-Linked Proteins”, Critical Reviews in Therapeutic Drug Carrier Systems, vol. 9, No. 3/4, pp. 249-304 (1992).
Favre, C. et al., “Epitope Mapping of Recombinant Human Gamma Interferon Using Monoclonal Antibodies”, Molecular Immunology, VOL. 26, NO. 1, pp. 17-25 (1989).
Francis, G.E. et al., “PEG-Modified Proteins”, Stability of Protein Pharmaceuticals, Part B: In Vivo Pathways of Degradation and Strategies for Protein Stabilization, edited by Ahern, T.J. and Mannin, M.C., Plenum Press, pp. 235-263 (1992).
Hopp, T.P. et al., “Prediction of Protein Antigenic Determinants from Amino Acid Sequences”, Proc. Natl. Acad. Sci., vol. 78, NO. 6, pp. 3824-3828 (1981).
Katre, N.V., “The Conjugation of Proteins with Polyethylene Glycol and Other Polymers”, Advanced Drug Delivery Reviews, vol. 10, pp. 91-114 (1993).
Khan, S.A. et al., “Polyethylene Glycol-modified Subtilisin Forms Microparticulate Suspensions in Organic Solvents”, Enzyme Microb. Technology, vol. 14, pp. 96-100 (Feb. 1992).
Masunaga, T. et al., “The Protease as a Cleasing Agent and Its Stabilization by Chemical Modification”, IFSCC, pp. 483-501, Yokohama.
Mitchinson, C., et al., “Protein Engineering of Disulfide Bonds in Subtilisin BPN”, Biochemistry, vol. 28, No. 11, pp. 4807-4815 (1989).
Monfardini, C. et al., “A Branched Monoethoxy Poly(ethylene glycol) for Protein Modification”, Biconjugate Chemistry, vol. 6, No. 1, pp. 62-69 (1995).
Nishimura, H. et al., “Improved Modification of Yeast Uricase with Polyethylene Glycol, Accompanied with Non-immunoreactivity Towards Anti-Uricase Serum and High Enzymic Activity”, Enzyme, vol. 26, pp. 49-53 (1981).
Nucci, M. L., et al., “Immunogenicity of Polyethylene Glycol-Modified Superoxide Dismutase and Catalase”, J. Free Radicals in Biology and Medicine, vol. 2, pp. 321-325 (1986).
Nucci, M.L. et al., “The Therapeutic Value of Poly(ethylene glycol)-modified Proteins”, Advanced Drug Delivery Reviews, vol. 6, pp. 133-149 (1991).
Ohta, M. et al., “Preparation of a Dextran-Protease Conjugate and its Application to Cosmetic Use”, KANEBO, Ltd., Cosmetics & Toiletries® Magazine, vol. 111, (1996).
Reay, P.A. et al., “Use of Global Amino Acid Replacements to Define the Requirements for MHC Binding and T Cell Recognition of Moth Cytochrome c (93-103)”, Journal of Immunology, vol. 152, NO. 8, pp. 3946-3957 (1994).
Ritz, H.L. et al., ȁ

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