Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-10-15
1999-06-01
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 436 94, C12Q 168, C12P 1934, G01N 3348
Patent
active
059087552
ABSTRACT:
In one aspect, described are methods of sequencing of polynucleotides using a sequential step procedure. The methods of the invention begin with the provision of a single-stranded polynucleotide template that is annealed with a primer, forming a template-primer complex. The methods of the invention can provide for adding discrete nucleotides by a polymerase to the template-primer complex, where the added nucleotides can be labeled. The methods of the invention can involve the identification of a polynucleotide or polynucleotides having a contiguous non-redundant string or a superimposed non-redundant string pattern. The detection of the presence of a non-redundant contiguous string can be used, for example, to identify a particular gene. Alternatively, for example, if the non-redundant contiguous string is not unique to a particular gene, the string can be used to form a DNA library that can then be searched, for example, with a second string. Similarly, a superimposed non-redundant string pattern can be used, for example, to identify a gene or to search a DNA library. Such strings can be used, for example, in annealing reactions and in computer searches of a database having a catalog of sequenced polynucleotides.
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Heaney Paul
Kumar Rajan
Burke William J.
Horlick Kenneth R.
Sarnoff Corporation
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