Sequencing method and apparatus

Details Sequencing method and apparatus Sequencing method and apparatus

2006-11-07

2006-11-07

Brusca, John S. (Department: 1631)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

C435S006120

Reexamination Certificate

active

07133782

ABSTRACT:
In a method of identifying an unknown nucleotide sequence using base addition, a sequence of bases is obtained from a template, a base in the sequence is identified as an unknown base, an “unknown” indicator is included in the sequence, and an output sequence is generated containing the unknown base indicator. The sequence of bases is obtained from the template by evaluation of a reporter and assigning the bases in accordance therewith. A determination is made as to whether the reporter is from a preceding cycle of base determination, and if the reporter is from a preceding cycle of base determination, the base assignation is discarded.

REFERENCES:
patent: 5302509 (1994-04-01), Cheeseman
patent: 5552278 (1996-09-01), Brenner
patent: WO 91/06678 (1991-05-01), None
patent: WO 93/05183 (1993-03-01), None
patent: WO 93/21340 (1993-10-01), None

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