Sequencing method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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Details

C435S091100, C435S091200, C436S094000, C536S023100, C536S024300, C536S024330

Reexamination Certificate

active

07056670

ABSTRACT:
Disclosed is a single molecule sequencing method comprising the steps of: a) attaching a molecule to a solid phase; b) incubating the molecule with a first composition comprising a first reporter moiety; c) detecting incorporation of said first reporter moiety; d) performing a reaction to eliminate said first reporter moiety; e) incubating the molecule with a second composition comprising a second reporter moiety; f) detecting incorporation of said second reporter moiety; characterized in that the first and second reporter moieties can be distinguished from each other.

REFERENCES:
patent: 5302509 (1994-04-01), Cheeseman
patent: 6136543 (2000-10-01), Anazawa et al.
patent: 6355435 (2002-03-01), Wilson et al.
patent: WO90/13666 (1990-11-01), None
patent: WO96/27025 (1996-09-01), None
patent: WO99/05315 (1999-02-01), None
patent: WO00/06770 (2000-02-01), None
patent: WO00/18956 (2000-04-01), None
patent: WO00/58507 (2000-10-01), None

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