Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2001-01-09
2004-08-03
Winkler, Ulrike (Department: 1648)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S005000, C435S006120, C435S091200, C536S023100, C536S023720, C536S024300
Reexamination Certificate
active
06770752
ABSTRACT:
FIELD OF THE INVENTION
The present invention relates to materials and methods for detection of HIV nucleic acids, in particular to probes and primers for detection of HIV in hybridization and amplification assays.
BACKGROUND OF THE INVENTION
The genome of the Human Immunodeficiency Virus (HIV) is highly heterogeneous and exhibits a mutation rate on the order of 10
−4
per base per generation. Combined with the rapid rate of viral propagation in infected individuals, this presents particular challenges for diagnostic nucleic acid amplification techniques which typically amplify a single conserved sequence within a target organism. Although a great deal of research activity has been directed to detecting HIV-1 in hybridization and amplification assays, such molecular assays have so far been limited by their inability to detect all genotypes with equal efficiency. Although a signal amplification system for the detection of HIV-1 type O has been reported, none of the currently available diagnostic nucleic acid amplification methods are able to detect this genotype of the virus.
SUMMARY OF THE INVENTION
The present invention provides primers and probes derived from the HIV-1 pol gene which facilitate detection and/or quantification of all presently known genotypes of HIV-1 (A-I and O). A single amplification primer pair according to the invention efficiently amplifies all known genotypes of HIV-1, which may then be detected in a single detection step using the detector probes and primers of the invention.
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Harris James M.
Hellyer Tobin J.
You Qimin
Becton Dickinson and Company
Kiang Allan M.
Winkler Ulrike
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