Sequences encoding PhzO and methods

Details Sequences encoding PhzO and methods Sequences encoding PhzO and methods

2007-03-13

2007-03-13

Patterson, Jr., Charles L. (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

Reexamination Certificate

active

10810904

ABSTRACT:
Th invention is directed to nucleic acid sequences which encode polypeptides having PhzO activity, namely, the ability to convert phenazine-1-carboxylic acid to a 2-hydroxylated phenazine, and isolated polypeptides having this activity. The invention is also directed to recombinant nucleic acid molecules, vectors, and host cells including the nucleic acid sequences as well as methods for producing and using the polypeptides, including expression in bacterial or plant cells to inhibit fungal pathogens.

REFERENCES:
patent: 5639949 (1997-06-01), Ligon et al.
Guo, H.H., et al. (2004), Proc. Natl. Acad. Sci., USA 101(25), 9205-9210.
Chin-A-Woeng, T.F.C. et al., “ Biocontrol by Phenazine-1-carboxamide-ProducingPseudomonas chlororaphisPCL1391 of Tomato Root Rot Caused byFusarium oxysporumf. sp.radicis-lycopersici,” Mol. Plant-Microbe Interact.(1998) 11(11):1069-1077.
Delaney, S.M et al., “phzO, a Gene for Biosynthesis of 2-Hydroxylated Phenazine Compounds inPseudomonas aureofaciens30-84,”J. of Bacteriology(2001) 183(1):318-327.
Duffner, F.M. and R. Müller, “A novel phenol hydroxylase and catechol 2,3-dioxygenase from the thermophilicBacillus thermoleovoransstrain A2: nucleotide sequence and analysis of the genes,”FEMS Microbiol. Lett.(1998) 161:37-45.
Galán, B., E. Diáz, M.A. Prieto, and J.L. Garcia, “Functional Analysis of the Small Component of the 4-Hydroxyphenylacetate 3-Monooxygenase ofEscherichia coliW: a Prototype of a New Flavin:NAD(P)H Reductase Subfamily,”J. Bacteriol.(2000) 182(3):627-636.
Gibello, A., M. Suárez, and J.L. Allende, “Molecular Cloning and Analysis of the Genes Encoding the 4-hydroxyphenylacetate hydroxylase fromKlebsiella pneumoniae,” Arch. Microbiol.(1997) 167:160-166.
Hübner, A., C.E. Danganan, L. Xun, A.M. Chakrabarty, and W. Hendrickson, “Genes for 2,4,5-trichlorophenoxyacetic Acid Metabolism inBurkholderia cepaciaAC1100: Characterization of thetftCandtftDGenes and Locations of thetftoperons on Multiple Replicons,”Applied and Environmental Microbiology(1998) 64(6):2086-2093.
Mavrodi, D.V. et al., “A Seven-Gene Locus for Synthesis of Phenazine-1-Carboxylic Acid byPseudomonas fluorescens, 2-79,”J. of Bacteriology(1998) 180(9):2541-2548.
Pierson III, L.S. and L.S. Thomashow, “Cloning and heterologous expression of the phenazine biosynthetic locus fromPseudomonas aureofaciens30-84,” (1992)Mol. Plant-Microbe Interact. (5):330-339.
Prieto, M.A. and J. L. Garcia, “Molecular Characterization of 4-Hydroxylphenylacetate 3-Hydroxylase ofEscherichia coli,” Journal of Biological Chemistry(1994) 269(36):22823-22829.
Takizawa, N., H. Yokoyama, K. Yanagihara, T. Hatta, and H. Kiyohara, “A Locus ofPseudomonas pickettiiDTP0602,had, That Encodes 2,4,6-Trichlorophenol-4-Dechlorinase with Hydroxylase Activity, and Hydroxylation of Various Chlorophenols by the Enzyme,”J. Ferment. Bioeng.(1995) 80(4):318-326.
Xun, L., and E.R. Sandvik, “Characterization of 4-Hydroxyphenylacetate 3-Hydroxylase (HpaB) ofEscherichia colias a Reduced Flavin Adenine Dinucleotide-Utilizing Monooxygenase,”Applied and Environmental Microbiology(2000) 66(2):481-486.

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