Sequence-specific methylation of ribonucleic acid

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification

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435 911, 435445, 514 44, 536 231, C07H 2102, C12P 1934, A01N 4304

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active

059727055

ABSTRACT:
The invention features nucleic acid molecules and methods for use in site-specific methylation of ribonucleotides. The methods of the invention can be used to modulate RNA folding, RNA processing, RNA cleavage, and other processes involving sequence-specific recognition of RNA sequences, as well as for promoting RNA stability.

REFERENCES:
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Cavaille et al., "Targeted ribose methylation of RNA in vivo directed by tailored antisense RNA guides", Nature 383:732-735, 1996.
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Nicoloso et al., "Intron-encoded Antisense Small Nucleolar RNAs: The Characterization of Nine Novel Species Points to Their Direct Role as Guides for the 2'-0-ribose Methylation of rRNAs", J. Mol. Biol 260, 178-195, 1996.
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Tycowski et al., "A small nucleolar RNA requirement for site-specific ribose methylation of rRNA in Xenopus", Proc. Natl. Acad. Sci. USA 93:14480-14485, 1996.
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