Sequence specific DNA binding by p53

Details Sequence specific DNA binding by p53 Sequence specific DNA binding by p53

1994-09-01

1999-09-21

Huff, Sheela

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 723, 436 63, 436 64, 536 231, 536 241, C12Q 168, G01N 33574, G01N 3348, C07H 2102

Patent

active

059552638

ABSTRACT:
Specific sequences in the human genome are the sites of strong binding of wild-type p53 protein, but not mutant forms of the protein. These sequences are used diagnostically to detect cells in which the amount of wild-type p53 is diminished. The sequences can also be used to screen for agents which correct for loss of wild-type p53 to DNA in cancer cells.

REFERENCES:
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Bargonetti et al., "Wild-Type But Not Mutant p53 Immunopurified Proteins Bind to Sequences Adjacent to the SV40 Origin of Replication," Cell 65:1-9 (1991).
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