Sequence-directed DNA-binding molecules compositions and methods

Details Sequence-directed DNA-binding molecules compositions and methods Sequence-directed DNA-binding molecules compositions and methods

1993-12-20

1996-11-26

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 723, 536 231, 935 76, 935 77, C12Q 168, C12N 1500, G01N 33574, C07H 2102

Patent

active

055784445

ABSTRACT:
The present invention defines a DNA:protein-binding assay useful for screening libraries of synthetic or biological compounds for their ability to bind DNA test sequences. The assay is versatile in that any number of test sequences can be tested by placing the test sequence adjacent to a defined protein binding screening sequence. Binding of molecules to these test sequence changes the binding characteristics of the protein molecule to its cognate binding sequence. When such a molecule binds the test sequence the equilibrium of the DNA:protein complexes is disturbed, generating changes in the concentration of free DNA probe. Numerous exemplary target test sequences (SEQ ID NO:1 to SEQ ID NO:600) are set forth. The assay of the present invention is also useful to characterize the preferred binding sequences of any selected DNA-binding molecule.

REFERENCES:
patent: 4257774 (1981-03-01), Richardson et al.
patent: 4270924 (1981-06-01), Crooke et al.
patent: 5071773 (1991-12-01), Evans et al.
patent: 5096815 (1992-03-01), Ladner et al.
patent: 5306619 (1994-04-01), Edwards et al.
Ray, R., et al., "Mithramycin Blocks Protein Binding and Function of the SV40 Early Promoter," J. Clin. Invest. 83:2003-2007 (1989).
Snyder, R. C., et al., "Mithramycin Blocks Transcriptional Initiation of the c-myc P1 and P2 Promoters," Biochemistry 30:4290-4297 (1991).
Van Dyke, M. W., and Dervan, P. B., "Chromomycin, Mithramycin, and Olivomycin Binding Sites on Heterogeneous Deoxyribonucleic Acid --Footprinting with (Methidiumpropyl-EDTA)ironIII)," Biochemistry 22:2273-2377 (1983).
Davison, A. J., and Scott, J. E., "The Complete DNA Sequence of Varicella-Zoster Virus," J. Gen. Virol. 67:1759-1816 (1986).
Hanvey, J. C., et al., "Site-Specific Inhibition of EcoR1 Restriction/Modification Enzymes by a DNA Triple Helix," Nuc. Acids Res. 18(1):157-161 (1990).
Hobson, K., et al., "Use of DNA-Protein Interaction to Isolate Specific DNA Sequences," Anal. Biochem. 193:220-224 (1991).
McGeoch, D. J., et al., "The Complete DNA Sequence of the Long Unique Region in the Genome of Herpes Simplex Virus Type 1," J. Gen. Virol. 69:1531-1574 (1988).

Affiliated with

Also associated with

Rating

Sequence-directed DNA-binding molecules compositions and methods does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Sequence-directed DNA-binding molecules compositions and methods, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Sequence-directed DNA-binding molecules compositions and methods will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1972094