Sequence-directed DNA-binding molecules compositions and methods

Details Sequence-directed DNA-binding molecules compositions and methods Sequence-directed DNA-binding molecules compositions and methods

1995-06-07

1998-04-14

Guzo, David

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435691, 4351723, 4353201, 536 241, 935 36, 935 39, C12P 2102, C12N 1567, C07H 2104

Patent

active

057389900

ABSTRACT:
The present invention defines an assay useful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA test sequences. The assay is also useful for determining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. Also described herein are potential applications of the assay, including: 1) the detection of lead compounds or new drugs via the mass screening of libraries of synthetic or biological compounds (i.e., fermentation broths); 2) the design of sequence-specific DNA-binding drugs comprised of homo- or hetero-meric subunits of molecules for which the sequence specificity was determined using the assay; and 3) the use of molecules for which sequence specificity was determined using the assay as covalently attached moieties to aid in the binding of nucleic acid or other macromolecular polymers to nucleic acid sequences.

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